Figure 4.

Supramolecular recruitment of fluorescent monomers occurs site‐selectively. To demonstrate site‐selective incorporation of monomers onto the DNA platform using origami‐to‐monomer FRET, the DNA origami structures were adapted to include 15 Cy3 fluorophores positioned at either 6 nm (a) or 39 nm (b) from the 15 handles for DNA‐monomer incorporation. To pre‐assemble supramolecular polymers, 4 nM of each DNA origami variant was incubated with a 2.5 μM pre‐annealed supramolecular polymer mixture of inert disc 1 containing 5 % DNA‐disc 2 for 1 h at 18 °C. After incubation, 250 nM Cy5‐disc 5 was added directly to the reaction mixtures. Time‐resolved FRET measurements in the absence (gray curves) or presence (red curves) of 5 % DNA‐disc 2 revealed an increase in FRET when the Cy3 fluorophores are positioned at 6 nm from the DNA‐monomer incorporation sites, and no increase at 39‐nm spacing. Data is represented as mean ± s.d. of three independent experiments.