Figure 5.

Antibody‐prothrombin complexes compete with coagulation factors on phospholipid binding sites. Biotinylated activated factor X (FXa) was incubated with lipospheres and competition with antibody‐beta2‐glycoprotein I (β2GPI) or antibody‐prothrombin complexes was assessed with flow cytometry. Significant differences were determined between baseline FXa binding at 0 µg/mL and other concentrations of monoclonal antibodies using an unpaired Student’s t‐test (n = 3). *P‐value<0.05