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. 2021 Mar 3;29(4):721–730. doi: 10.1002/oby.23122

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© 2021 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society (TOS).

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Dopamine transporter (DAT) protein levels. Western blot quantification of DAT protein (normalized to β‐actin) from enriched membrane and cytosolic fractions of nucleus accumbens brain tissue. Normalized DAT protein in each group was expressed as a percentage of that of the control group. (A) Histological representation of DAT band density at ~150, 78, and 68 kDa in membrane fractions and (B) cytosolic fractions showed a reduced density of only the 68‐kDa DAT band in all groups compared with the control group. Representative blots showing DAT band density at 150, 78, and 68 kDa, as well as the 46‐kDa β‐actin band containing (C) membrane and (D) cytosolic samples. All data reflect the mean ± SEM (*P < 0.05; ***P < 0.001).