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Properties of Ox‐SLIM 9.. a) CW X‐band EPR peak‐to‐peak amplitude of trityl spin labels 4., 6., and 9. (50 μm in PBS‐buffer) at increasing concentration of cysteine‐free YopO C219A. b) CW X‐band EPR spectra of YopO Y588Tox/N624Tox (black) and YopO C219A (red) after labelling and workup. The spectra were recorded at 298 K in PBS‐buffer. c) EPR double integral intensity of Y588Tox/N624Tox in HeLa lysate, X. laevis oocyte lysate, and 5 mm sodium ascorbate monitored over time.
