Figure 1.

Amuc_1434* degradation of Muc2. (A) Western blot analysis of degradation of Muc2 by Amuc_1434* at different Amuc_1434* concentrations (loading protein were 0, 2.5, 5, 10 and 20 µg, respectively). (B) Enzyme-linked immunosorbent assay (ELISA) analysis of remaining Muc2 after Amuc_1434* degradation at 450 nm. Triplicate samples were measured and error bars represented standard deviation of the data. (C) Immunofluorescence analysis of the Muc2 degradation ability of Amuc_1434*. The membrane was shown in green with 3,30-dioctadecyloxacarbocyanine perchlorate (DIO). Muc2 was labeled in red. Scale bar = 50 µm.