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Characterization of ZmbZIP33 gene. (A) The length of amino acid encoded by ZmbZIP33 gene and its domain. (B) Relationships between ZmbZIP33 and its homologs. Phylogenetic tree was generated using MEGA6 version (C) The construct pGBKT7-ZmbZIP33 was transformed into yeast strain AH109 and checked on SD/Trp and SD/Trp-/His-/Ade-/X-α-gal plates. pGBKT7-53 and pGADT7-T were used as a positive control, and pGBKT7 vector was used as a negative control.
