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. 2021 Apr 1;12:629903. doi: 10.3389/fpls.2021.629903

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Copyright © 2021 Cao, Lu, Wang, Zhang, Zhang, Fan, Cao, Wei, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of ABA sensitivity and drought resistance of ZmbZIP33 under drought stress. (A) Forty percent of PEG stress on BMV and BVM-ZmbZIP33 maize seedlings for 4 and 6 days (DOD). The plants were then rewatered for 4 days (R4 D) and photographed. (B–E) Chlorophyll content, SOD activity, POD activity, and ABA content of BMV and BVM-ZmbZIP33 leaves were measured under drought stress and rewatering. (F) Scanning electron microscopy images of three levels of stomatal opening in BMV and BMV-ZmbZIP33. Bar = 20 μm. (G) Water loss assays for the leaves of BMV and BMV-ZmbZIP33 transgenic line were performed within 5 h. Mean values and standard errors (bar) were shown from five independent experiments. Independent t-tests for equality of means demonstrated that there was significant difference between control and treatment (*P value < 0.05).