Skip to main content
. 2021 Apr 1;12:629903. doi: 10.3389/fpls.2021.629903

Figure 8.

Figure 8

Interaction analysis of ZmbZIP33, ZmPP2C7, ZmSRK2E, and ZmPYL10 and their expression patterns under drought stress and rewateing. (A) Fusion proteins were transiently expressed under the control of CaMV35S promoter in tobacco leaves and were subsequently observed under a laser scanning confocal microscope. Bars = 25 μm. (B) The pGADT7-T+pGBKT7-Lam and pGADT7-T+pGBKT7-53, as negative and positive controls, respectively. (C) The nYFP and cYFP represent pSAT4-nEYFP and pSAT4-cEYFP vectors, respectively. When an interaction relationship was verified, DAPI (nuclear marker) staining was selected. The interaction signal of ZmPYL10-cY and ZmPP2C-nY coincided with DAPI, indicating an interaction within the nucleus. (D) The expressions of ZmPP2C7, ZmSRK2E, and ZmPYL10 in BMV and BMV-ZmbZIP33 under drought stress for 0 days (0 DOD) and 4 days (4 DOD), and rewatering for 4 days (R4D) were measured. And the response patterns of ZmPP2C7, ZmSRK2E, and ZmPYL10 to drought stress (PEG-24 h), rewatering (R-24 h) and ABA (ABA-24 h, 5 μmol/L) treatments for 24 h were measured. Using maize 18S as an internal control, the relative expression was determined by 2−ΔΔCt0h to calculate.