FIGURE 1.

The Bradi1g75310 (BdCYP711A29) gene is transcriptionally induced during FHB and following DON treatment. Relative quantification of Bradi1g75310 transcripts in the Bd21-3 (WT) ecotype of B. distachyon following F. graminearum infections or following DON treatment. (A) Bradi1g75310 expression level (fold-change. log₂) following point infection with the FgDON⁺ (solid line) or with the FgDON^– (dashed line) strain of F. graminearum compared to mock treatment. (B) Bradi1g75310 expression level (fold-change. log₂) following DON treatment compared to mock treatment. The relative quantity of the Bradi1g75310 transcripts compared to mock condition was calculated using the comparative cycle threshold (Ct) method (2^–ΔΔCt). The B. distachyon UBC18 and ACT7 genes (Bradi4g00660 and Bradi4g41850) were used as endogenous controls to normalize the data for differences in input RNA between the different samples. Mean of three independent biological replicates ± standard deviation. Asterisks indicate significant differences between conditions (Student’s t-test p value < 0.05).