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. 2021 Jan 13;45(3):612–622. doi: 10.1002/cbin.11544

Figure 3.

Figure 3

Wear particles regulate autophagy by increasing the expression of Netrin‐1. (a) Western blot detection of Netrin‐1 expression in RAW 264.7 cells with or without treatment with Ti wear particles. (b) The mRNA expression of Netrin‐1 in RAW 264.7 cells with or without treatment with Ti wear particles. (c) The protein expression of UNC5b and DCC in RAW 264.7 cells. (d) The mRNA expression of Atg5, Beclin1, Atg7, and Atg12 in RAW 264.7 cells with or without treatment with Ti wear particles and IgG, Netrin‐1, Unc5b, or DCC neutralizing antibodies. (e) The expression of LC‐3 and Beclin1 was detected by western blotting in RAW 264.7 cells with or without treatment with Ti wear particles and IgG, Netrin‐1, Unc5b, or DCC neutralizing antibodies. (f) The mRNA expression of Atg5, Beclin1, Atg7, and Atg12 in RAW 264.7 cells with or without treatment with recombinant Netrin‐1. (g) The expression of LC‐3 and Beclin1 was detected by western blotting in RAW 264.7 cells with or without treatment with recombinant Netrin‐1. mRNA, messenger RNA