Figure 5.

(a) Netrin‐1 significantly promotes osteoclastogenesis. The expression of Trap was detected by western blotting in RAW 264.7 cells with or without treatment with Ti wear particles and IgG, Netrin‐1, or Unc5b neutralizing antibodies. (b) The expression of Trap and Beclin1 was detected by western blotting in RAW 264.7 cells with or without treatment with recombinant Netrin‐1 and 3‐MA. (c) The expression of Trap, p‐ERK and ERK was detected by western blotting in RAW 264.7 cells with or without treatment with recombinant Netrin‐1 and U0126. (d) Pit resorption assay for the effect of Netrin‐1 on osteoclast differentiation. IgG, immunoglobulin G