Table 1.
Proton (1H) and carbon (13C; italic) NMR chemical shifts of the O‐antigen of F. nucleatum ATCC 51191 (600 MHz, 25 °C, 550 μL of D2O, neutral pH). The chemical shifts of the methyl of the acetyl groups were at about 1H/13C 2.1‐1.8/23 ppm.
|
Residue |
1 |
2 |
3 |
4 |
5 |
6 |
|---|---|---|---|---|---|---|
|
A’ →3)‐α‐d‐FucpNAc4N‐(1→ |
5.32 |
4.02 |
4.20 |
3.82 |
4.27 |
1.24 |
|
98.2 |
48.9 |
75.2 |
56.0 |
64.1 |
16.2 |
|
|
A →3)‐α‐d‐FucpNAc4NAc‐(1→ |
5.29 |
4.13 |
4.09 |
4.27 |
4.17 |
1.04 |
|
98.4 |
50.1 |
74.6 |
54.3 |
67.3 |
16.5 |
|
|
B’ →4)‐β‐d‐GlcpNAc3NAlaA‐(1→ |
4.71 |
3.84 |
4.08 |
3.78 |
3.78 |
|
|
103.9 |
54.5 |
54.0 |
79.1 |
79.1 |
176.5 |
|
|
B →4)‐β‐d‐GlcpNAc3NAlaA‐(1→ |
4.64 |
3.67 |
4.06 |
3.73 |
3.73 |
|
|
102.8 |
55.2 |
54.0 |
79.3 |
79.3 |
176.5 |
|
|
C’ →4)‐β‐d‐GlcpNAcA‐(1→ |
4.47 |
3.65 |
3.73 |
3.69* |
3.75 |
|
|
102.0 |
56.9 |
75.5 |
78.1* |
78.0 |
176.5 |
|
|
C →4)‐β‐d‐GlcpNAcA‐(1→ |
4.46 |
3.63 |
3.71 |
3.67* |
3.75 |
|
|
102.9 |
56.7 |
75.5 |
77.9* |
78.0 |
176.5 |
|
|
Ala |
– 172.6 |
3.98 50.2 |
1.43 18.1 |
*C4 and C’4 attribution can be exchanged.