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. 2020 Nov 30;22(6):1012–1019. doi: 10.1002/cbic.202000574

Figure 3.

Figure 3

Screening of cyclic MIF(47–56) analogues for their inhibitory potential in vitro and characterization of their conformational properties. A) Overview of the screened cyclic MIF(47–56) analogues MIF(cyclo0), MIF(cyclo4), MIF(cyclo6), and MIF(cyclo10) and comparison to the linear parent peptide. The N‐like loop sequence 47–56 is highlighted in orange; amino acid sequences are depicted by the one‐letter code; cyclization through the disulfide bridge is indicated. B) Transwell‐based PBMC chemotaxis assay using MIF as the chemoattractant (n=4–11, mean±SD). Statistical significance is indicated: * P<0.05, ** P<0.01, ns=not significant. C) Circular dichroism (CD) spectra between 190 and 250 nm of the cyclic MIF(47–56) analogues in comparison to the linear peptide. Spectra were recorded three times, averaged, smoothed and are depicted as mean residue ellipticity (MRE). The inset is a close‐up of the spectra over the wavelength range 210–235 nm.