Figure 4.

MIF‐derived cyclic peptide MIF(cyclo10) exhibits high proteolytic stability in human blood plasma. MIF(cyclo10) and the linear peptide MIF(47–56) were incubated in human plasma at 37 °C for the indicated time intervals, intact peptides were quantitated by C₁₈ HPLC, and their molecular weights were verified by MALDI‐MS (see also Figure S2). The data shown are from three independent incubations, and error bars indicate mean±SD. Recovered intact peptide (% of total) is plotted over the various incubation time points. The inset shows the mass spectrum of the HPLC‐isolated peptide fraction after 40 h of incubation with plasma.