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. 2020 Nov 30;22(6):1012–1019. doi: 10.1002/cbic.202000574

Figure 5.

Figure 5

The MIF‐derived cyclic peptide MIF(cyclo10) blocks leukocyte adhesion to carotid arteries in vivo/ex vivo. A) Scheme indicating the experimental set‐up of the ex vivo/in vivo treatment regimen and leukocyte adhesion protocol in whole carotid arteries. B)–D) MPM analysis of adherent leukocytes after treatment with MIF(cyclo10). B)‐C) Example MPM images of adherent leukocytes to carotid arteries after treatment (red) or vehicle (green); B) 3D acquisition of a z‐stack series; C) representative 2D image; scale bar: 20 μm. D) Quantification of adherent leukocytes after treatment with MIF(cyclo10). Adherent cells under flow were quantified by MPM in situ. The data shown are mean±SD and are derived from four independent MIF(cyclo10)‐treated and four saline‐treated mice. Adhesion data were obtained on five (MIF(cyclo10)) and seven (saline) independent carotid arteries. Four of the prepared carotids did not inflate and could not be used for the adhesion experiments. Statistical significance is indicated: * P<0.05.