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. 2021 Jan 5;45(3):623–632. doi: 10.1002/cbin.11507

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© 2020 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Overexpression of CHRDL2 promoted osteosarcoma cell proliferation and mobility. (a) The efficiency of CHRDL2 overexpressed lentivirus (lv‐CHRDL2) was detected by PCR. (b) The efficiency of lv‐CHRDL2 was detected by western blot. (c) CCK8 assays were performed to detect the effects of CHRDL2 overexpression on osteosarcoma cell proliferation. (d) Colony formation assays were performed to detect the effects of CHRDL2 overexpression on osteosarcoma cell colony formation. (e) Wound healing assay was used to detect the effects of CHRDL2 overexpression on osteosarcoma cell migration. (f) Transwell assay was used to detect the effects of CHRDL2 overexpression on osteosarcoma cell invasion. *p < .05; **p < .01. CCK‐8, cell counting kit‐8; CHRDL2, chordin‐like 2; PCR, qRT‐PCR, polymerase chain reaction