FIGURE 4.

The lateral anterior optic tubercle (L‐AOTU) consists of four columns. (a) The anterior optic tubercle (AOTU) is located near the frontal surface of the brain at the side of the mushroom (MB). (b) The AOTU consists of the medial AOTU (M‐AOTU) and the lateral AOTU (L‐AOTU) and locates near the frontal surface of the brain. Imaging of anti‐discs large (DLG) stained neuropils (gray) reveals clear structural boundaries, including the vertical strata of the L‐AOTU. (b1) Each color reveals a demarcated substructure in the L‐AOTU. (c) Volume models of four columns in the L‐AOTU. (d1–d4) Expression of column‐specific Gal4 and LexA drivers in input MT neurons that were visualized with mCD8::GFP. (d5–d8) High‐magnification image in the L‐AOTU of column‐specific input MT neurons (boxed inset). (e1–e4) Expression of column‐specific Gal4 and LexA drivers expressed in output TB neurons that were visualized with mCD8::GFP. (e5–e8) High‐magnification image in the L‐AOTU of column‐specific output TB neurons (boxed inset). Brain structures immunostained with anti‐DLG antibodies (gray in a and b; blue in d and e). Images are frontal views of confocal projections of several adjacent optical sections unless otherwise specified. Scale bars: 20 μm [Color figure can be viewed at wileyonlinelibrary.com]