Figure 3.

CD3 cells are mainly located peritumorally in the orthotopic mEERL95 tumors. a: Example of the algorithmic detection of CD3 and E-cadherin immunostaining signal on frozen sections from orthotopic mEERL95 tumors. The epithelial-specific E-cadherin and nuclear DAPI signals are used to accurately define the peritumoral and intratumoral areas of the sample. b: Overview of a whole tumor section showing that CD3 T-cells are preferentially found in the peritumoral area. (inset): Quantification of the ratio between CD3 T-cells detected in the peritumoral area versus CD3 T-cells detected in the intratumoral area in PBS treated mice (n = 4)