Fig 3. Suppression of thorax defects and locomotion deficits.
(A) Time window targeted by light in experiments with PINK1B9 flies. Illumination of pupal and adult stages prevented lethality observed upon Opto-dRET signaling in earlier stages (e.g., Opto-dRETMEN2B flies were grown at 18°C to prevent lethality during development; see Main Text). (B) Percentage of flies with a degenerate thorax phenotype. Representative bright field thorax images shown on the right. Hollow thorax is highlighted by the red arrow. (C) Climbing ability of flies. “M2B” denotes Opto-dRETMEN2B. PINK1 “+” denotes the WT gene. For B and C, counts ± SE for the indicated number of flies (n) is given. Percentages sharing the same label are not significantly different (Fisher’s exact test, p>.04). Continuous light intensity was 320 μW/cm2 for the duration shown in A.