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. 2021 Apr 15;17(4):e1009479. doi: 10.1371/journal.pgen.1009479

Fig 4. Improved mitochondrial structure and function.

Fig 4

(A) ATP content in fly thoraces from PINK1B9 flies at the indicated conditions (normalized to the mean for control flies shown as bar 1). (B-E) Representative TEM images of thoracic indirect flight muscles. Arrow heads indicate mitochondria that are either electron dense (B: controls, E: illuminated PINK1B9 Opto-dRET flies) or malformed with disintegrated cristae (C: PINK1B9 flies, D: PINK1B9 Opto-dRET flies in the absence of light). Scale bar: 2 μm. (F) Analysis of mitochondrial density in TEM images. “M2B” denotes Opto-dRETMEN2B. PINK1 “+” denotes the WT gene. In A, the number of analyzed flies is given (at least three independent experiments) and bars sharing the same label are not significantly different (ANOVA/Bonferroni corrected t-tests of means, p>.04). In F, the number of analyzed micrographs is given (at least three independent experiments) and bars sharing the same label are not significantly different (ANOVA/Bonferroni corrected t-tests of means, p>.04). Continuous light intensity was 320 μW/cm2 for the duration shown in Fig 3A.