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. 2021 Apr 15;16(4):e0250232. doi: 10.1371/journal.pone.0250232

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© 2021 Zhang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Insulin secretion from MIN6-K8 cells (A) (n = 4), isolated mouse islets (B, C) (n = 4), and human islets (D) (n = 4) with or without PTX or YM254890 pretreatment. (E) Efficacy of Gα_i/o knockdown in MIN6-K8 cells (n = 4). (F) NMU-induced suppression of GSIS after Gα_i/o knockdown in MIN6-K8 cells (n = 4). (G) mRNA levels of Gnao1 or Gnai2 in MIN6-K8 cells after respective knockdown of Gnai2 or Gnao1 (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significant.