Figure 10.

Immunohistochemical analysis for aminopeptidase P (JG12) expression in the kidney tissue evaluated 90 days after ischemia/reperfusion insult (IRI) on day 30. (A) Bar graph of JG12 expression values. (B) Representative photomicrographs of immunostaining for JG12 in the renal cortex from an IRI, VDD+IRI and VDD+IRI+R rat (×400). Note that the restauration of vitamin D levels ameliorated the expression of JG12 in the VDD+IRI+R rats. Data are mean ± SEM. ^cp < 0.05, vs. IRI; ^ep < 0.01 vs. VDD+IRI. VDD, vitamin D deficiency; R, vitamin D replacement. Diet protocol: IRI, standard diet for 120 days; VDD+IRI, vitamin D-free diet for 120 days; and VDD+IRI+R, vitamin D-free diet in the first 30 days and standard diet in the last 90 days.