Fig 1. An elevated percentage of CD4⁺ T cells in peripheral blood that express the Th17 phenotype are found in SE-expressing mice.

Representative multicolor flow cytometric scatter plots before (A) and after (B) inoculation of B6.DR1 mice with P. gingivalis. Square gates represent the percentage of DAPI⁺CD45⁺CD3⁺CD4⁺ cells among the PBMCs that are expressing IL-17F. Peripheral blood samples from WT (B6) or B6.DR1 mice were analyzed by flow cytometry (C) before (Day 0) and at three times post oral inoculation (vertical grey bars) with P. gingivalis. Events are gated on DAPI⁺CD45⁺CD3⁺CD4⁺ cells, and results are expressed as the percentage of these cells among PBMCs that are of the Th17 phenotype. Stats: t-test.