Figure 1.

CV38-142 binds and cross-neutralizes SARS-CoV-2 and SARS-CoV

(A) CV38-142 Fab binds to RBDs from human, bat, and pangolin sarbecoviruses with generally similar affinities. Binding kinetics were measured by biolayer interferometry (BLI) with RBDs on the biosensor and Fab in solution. Concentrations of Fab serial dilution are shown in the inset in the lower right panel. The association and disassociation were recorded in real time (s) on the x axis with binding response (nm) on the y axis with colored lines. Disassociation constant (K_D) values were obtained by fitting a 1:1 binding model. The fitted curves are represented by the dashed lines (black).

(B) CV38-142 neutralizes both SARS-CoV-2 and SARS-CoV, while its Fab counterpart barely neutralizes the two pseudotype viruses at the highest concentrations tested in the same neutralization assay. The IgG half-maximal inhibitory concentration (IC₅₀) values (3.46 μg/mL for SARS-CoV-2 and 1.41 μg/mL for SARS-CoV) were determined using Prism software (version 8.4.3). Error bars indicate standard deviation (SD) of at least two biological replicates.