Figure 1.

spam mutations restore polarity to pam-1 embryos (A) DIC images show that wild-type embryos exhibit pseudocleavage (arrowhead; n = 19) during axis polarization and divide asymmetrically (n = 44). Many pam-1(or347) embryos do not exhibit pseudocleavage (n = 39) or an asymmetric division (n = 79), but the presence of a suppressor mutation improves both pseudocleavage (arrowhead) (lz4 n = 28; lz5 n = 46; lz6 n = 20) and the asymmetric division (lz4 n = 54; lz5 n = 85; lz6 n = 20) in many embryos. Anterior to the left. (B) Quantification of DIC time-lapse data. Chi square analysis was used to compare pam-1 embryos to each suppressed strain. *P < 0.05; **P < 0.01; ***P < 0.001