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. 2021 Mar 3;11(4):jkab063. doi: 10.1093/g3journal/jkab063

Figure 2.

Figure 2

spam-1(lz5) maps to LG II and has a missense mutation in wee-1.3. (A) Plotting the allele frequency of known Hawaiian SNPs against chromosomal position reveals an ∼6.5 Mb region on LG II that contains spam-2(lz5). Allele frequencies were obtained by WGS of mapping lines generated by crossing pam-1(or347); spam-2(lz5) worms to Hawaiian males. A missense variant was detected in the gene wee-1.3. Red line = LOESS regression trendline. The approximate position of wee-1.3 is marked by a red arrowhead. (B) Sanger sequencing was used to confirm the presence of the wee-1.3 missense mutation in our strains. The wee-1.3(lz5) allele has a G1459A(E487K) mutation (*). The CRISPR recreated wee-1.3(syb1738) strain had the identical change (*), as well as some silent mutations( ^) that were introduced to prevent recutting by Cas9; A1461G and G1467C are shown here. All sequences are from strains in the pam-1(or403) background.