Table 5.
Genotype | Number of NMY-2 foci |
Size of NMY-2 foci (µm2) |
||
---|---|---|---|---|
-700 seconds | -500 seconds | -700 seconds | -500 seconds | |
+/+ | 39.33 ± 5.49 | 29.17 ± 5.89 | 3.62 ± 0.22* | 2.93 ± 0.43* |
pam-1(or403) | 29.90 ± 2.57 | 21.30 ± 5.52 | 2.61 ± 0.30 | 1.62 ± 0.21 |
pam-1(or403); wee-1.3(lz5) | 29.75 ± 1.76 | 27.62 ± 4.47 | 2.69 ± 0.48 | 2.23 ± 0.53 |
Data gathered from confocal images at 700 and 500 seconds prior to pro-NEBD (‘see Materials and methods’). Averages and standard error shown for wild-type (n = 8), pam-1 (n = 10), and pam-1; wee-1.3 (n = 8). T-Tests to compare each strain to pam-1 shows no difference in the number of foci, but a difference in area between wild-type and pam-1.
P < 0.05