Figure 5.

Diverse TCRαβ repertoire and promiscuous TCRα-TCRβ pairing within B7/N₁₀₅-specific CD8⁺ T cells

(A and B) B7/N₁₀₅-specific CD8⁺ T cells were enriched by TAME and then single-cell sorted for TCRαβ analysis. Pie charts of TRBV and TRAV gene usage in B7/N₁₀₅⁺CD8⁺ T cells in (A) COVID-19 (n = 4) and (B) pre-pandemic donors (n = 4). All COVID-19 patients were from convalescent samples with one exception, where 5 out of 36 TCR clonotypes for Donor CA12 were from the acute time point. Segments shown by the same color represent TCRαβ clonotypes with the same V segment usage but different CDR3 sequences.

(C) Circos plots of TRBV and TRAV clonotype pairings; left arch and segment color indicate TRBV usage, and right outer arch color depicts TRAV usage.

(D) Bubble plot showing the distribution (number of donors and frequency) of TRBV/TRAV gene usage in COVID-19 patients.

(E) Dominant clonotypes identified in HLA-B^∗07:02 donors specific to B7/N₁₀₅⁺CD8⁺ T cells. ND, not determined.

See also Table S2.