Table 1. Number of proteins found in proteomes.
Result of profile HMM searches of MCAfunc and PLAC8 domains in proteomes of 25 taxa across viridiplantae. Number of proteins retaining the MCAfunc or PLAC8 domains (E value < 10−3) are listed and arranged following the Tree of Life (see S2, S7, and S8 Appendices).
| Vernacular | ID | Taxon | No of proteins with | |
|---|---|---|---|---|
| name | MCAfunc | PLAC8 | ||
| Chlorophytes | CHLRE | Chlamydomonas reinhardtii | 0 | 12 |
| Chlorophytes | VOLCA | Volvox carteri f. nagariensis | 0 | 7 |
| Charophytes | KLENI | Klebsormidium nitens | 2 | 13 |
| Charophytes | CHABU | Chara braunii | 8 | 12 |
| Bryophytes | MARPO | Marchantia polymorpha | 9 | 13 |
| Bryophytes | MapoRu | Marchantia polymorpha subsp. ruderalis | 9 | 7 |
| Bryophytes | PHYPA | Physcomitrella patens | 9 | 23 |
| Lycophytes | SELML | Selaginella moellendorffii | 17 | 15 |
| Gymnosperm | CMI | Cycas micholitzii | 1 | 12 |
| Gymnosperm | TBA | Taxus baccata | 7 | 10 |
| Angiosperm | AMBTC | Amborella trichopoda | 7 | 9 |
| Angiosperm | MUSAM | Musa acuminata subsp. malaccensis | 9 | 26 |
| Angiosperm | ORYSJ | Oryza sativa subsp. japonica | 29 | 23 |
| Angiosperm | MAIZE | Zea mays | 11 | 25 |
| Angiosperm | SORBI | Sorghum bicolor | 13 | 19 |
| Angiosperm | AQUCA | Aquilegia coerulea | 10 | 10 |
| Angiosperm | VITVI | Vitis vinifera | 7 | 20 |
| Angiosperm | POPTR | Populus trichocarpa | 12 | 26 |
| Angiosperm | MEDTR | Medicago truncatula | 10 | 22 |
| Angiosperm | CUCSA | Cucumis sativus | 5 | 10 |
| Angiosperm | GOSRA | Gossypium raimondii | 14 | 29 |
| Angiosperm | BRAOL | Brassica oleracea var. oleracea | 7 | 29 |
| Angiosperm | ARATH | Arabidopsis thaliana | 5 | 20 |
| Angiosperm | ERYGU | Erythranthe guttata | 9 | 27 |
| Angiosperm | SOLLC | Solanum lycopersicum | 7 | 19 |
| sum | 217 | 438 | ||
The number of MCAfunc domain proteins within a proteome varied between species. In charophytes, Klebsormidium nitens had two proteins, but Chara braunii eight. In angiosperms, monocots possessed generally higher numbers between nine and 29, whereas dicots five to 14 per species. The number of PLAC8 domain proteins was between seven and 30 per species, the lowest in the liverwort Marchantia polymorpha subsp. ruderalis and the highest in Brassica oleracea var. oleracea. The more complete liverwort proteome of Marchantia polymorpha (BUSCO 96.7% in UniProt) had 14 PLAC8 genes. The low number in M. polymorpha subsp. ruderalis (BUSCO 91.3%) might be explained by the incompleteness of its proteome. The full MCA protein with both of MCAfunc and PLAC8 domains was not found in charophytes. In streptophytes, at least one and up to three full MCA proteins were found per species.