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. 2021 Feb 3;217(4):iyab013. doi: 10.1093/genetics/iyab013

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Published by Oxford University Press on behalf of Genetics Society of America 2021. This work is written by US Government employees and is in the public domain in the US.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Western blot analysis of Hsp42, Btn3 and Sis1 levels in incurable mutants. (A) Hsp42-HA and Btn3-HA were expressed from their own promoters on CEN plasmids in log phase cells and were visualized by staining with anti-HA tag antibodies. Anti PGK staining was used as a loading control. Btn3 is 57.6 kDa; Hsp42 42.8 kDa; PGK1 44.7 kDa, and the HA tag adds 1.1 kDa. For Western blots 10 μg of protein per lane was loaded. (B) Anti-Sis1 staining of lysates of wild-type and Btn2-incurable mutants. Strains carried CEN plasmids expressing Btn2-HA and Hsp42-HA from their own promoters.