Figure 5.

Propagon/seed number of [URE3-1] in wild-type and Btn2-incurable mutants. Strains with [URE3-1] were plated on YPAD containing guanidine (3 mM in the top and bottom panels, 5 mM in the middle panel) to inhibit Hsp104 (Jung et al. 2002) and thus block prion propagation by preventing filament cleavage (Ness et al. 2002). Each of 10 colonies was resuspended in water and plated on SC-Ade, and the number of colonies forming is a measure of propagon number (Cox et al. 2003). Separate experiments with the same mutant are shown as separate “whisker” diagrams.