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60S ribosomal subunit deficiency prevents [URE3-1] curing. Mutants in genes encoding 60S ribosomal subunit proteins carrying [URE3-1] and overproducing Btn2p or Cur1p were examined as described in section Methods. The stability of [URE3] in the wild-type and mutant strains was tested before introduction of the CUR1/BTN2 overexpression plasmids and loss of [URE3] was <1% in all cases.
