Table 1.
Strains of Saccharomyces cerevisiae
| Strain | Parent | Genotype | Source |
|---|---|---|---|
| 1735 | MA116-8A | MATα his- ura2-60 [URE3-1] | (Lacroute 1971; Aigle and Lacroute 1975) |
| BEE1027 | 74D-694 | MAT a ura3 leu2 trp1 his3 ade1-14 [PIN+] [psi-] | (Chernoff et al. 1995) |
| BEE1021 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 [URE3-1] | (Brachmann et al. 2005) |
| 4591 | MATα his3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 [ure-o] | (Brachmann et al. 2005) | |
| BEE1190 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 hsp42::KanMX [URE3-1] | (Wickner et al. 2014) |
| BEE1192 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 ubr2::KanMX [URE3-1] | This study |
| BEE1251 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl21b::KanMX [URE3-1] | This study |
| BEE1272 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl4a::KanMX [URE3-1] | This study |
| BEE1318 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 ubr2::KanMX rpn4::URA3 [URE3-1] | This study |
| BEE1363 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl16b::KanMX [URE3-1] | This study |
| BEE1364 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl11b::KanMX [URE3-1] | This study |
| BEE1372 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rps14a::KanMX [URE3-1] | This study |
| BEE1378 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rps30b::KanMX [URE3-1] | This study |
| BEE1086 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 snc1::KanMX [URE3-1] | This study |
| BEE1096 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 snc2::KanMX [URE3-1] | This study |
| BEE1132 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 tlg2::KanMX [URE3-1] | This study |
| BEE1125 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 vps27::KanMX [URE3-1] | This study |
| BEE1219 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 vps35::KanMX [URE3-1] | This study |
| BEE1088 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 vps26::KanMX [URE3-1] | This study |
| BEE1012 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 btn2::TRP1 cur1::kanMX [URE3-1] | (Kryndushkin et al. 2008) |
| BEE1182 | BY245 | MATα his3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 [ure-o] | This study |
| BEE1200 | 5169-3D | MATα trp1 kar1 DAL5p-ADE2 leu2 ura3 Hsp104T160M—URA3 [ure-o] | This study |
| BEE1201 | 5169-7B | MATα trp1 kar1 DAL5p-ADE2 leu2 ura3 [ure-o] | This study |
| BEE1202 | 5168-9A | MATα trp1 kar1 DAL5p-ADE2 leu2 ura3 Hsp104T160M—URA3 [ure-o] | This study |
| BEE1203 | 5169-5A | MATα trp1 kar1 DAL5p-ADE2 leu2 ura3 [ure-o] | This study |
| BEE1407 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl21a::KanMX [URE3-1] | This study |
| BEE1319 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpn4::KanMX [URE3-1] | Emily Stroobant and Herman Edskes |
| BEE1103 | 779-6A | MATα kar1 ade2.1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3.52 [PSI+ strong] | |
| BEE1104 | 779-6A | MATα kar1 ade2.1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3.52 [PSI+ Sc4] | Mike Reidy |
| BEE1105 | 779-6A | MATα kar1 ade2.1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3.52 [PSI+ Sc37] | Mike Reidy |
| BEE1106 | 779-6A | MAT a kar1 ade2.1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3.52 [PSI+ LiebW] | Mike Reidy |
| BEE1107 | 779-6A | MAT a kar1 ade2.1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3.52 [PSI+ LiebS] | Mike Reidy |
| BEE1386 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-CAN1: CAN1 kar1 arg1::TRP1 lys2::LEU2 [URE3-1] | Morgan DeWilde and Herman Edskes |
| BEE1387 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-CAN1: CAN1 kar1 arg1::TRP1 lys2::LEU2 hsp42::KanMX [URE3-1] | This study |
| BEE1392 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-CAN1: CAN1 kar1 arg1::TRP1 lys2::LEU2 rpl4a::KanMX [URE3-1] | This study |
| BEE1395 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-CAN1: CAN1 kar1 arg1::TRP1 lys2::LEU2 rpl21b::KanMX [URE3-1] | This study |
| BEE1401 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-CAN1: CAN1 kar1 arg1::TRP1 lys2::LEU2 rps30b::KanMX [URE3-1] | This study |
| BEE1271 | BY241 | MAT a ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl4a::KanMX [ure-0] | This study |
| BEE1368 | BY241 | MATα ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl4a::KanMX [ure-0] | This study |
| BEE1370 | BY241 | MATα ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 [ure-0] | This study |
| BEE1417 | BY241 | MATa ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 rpl21b::KanMX rpn4::URA3 [URE3-1] | This study |
| 5991 | BY241 | MATa ura3 leu2 trp1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 tma10::kanMX | This study |
| 74-D694 | MATa kar1 ade1-14 his3 leu2 trp1 ura3 [psi-][PIN+] | (Chernoff et al. 1995) | |
| 6010 | BY241 | MATα ura3 leu2 trp1 his3::TRP1 P-DAL5: ADE2 P-DAL5: CAN1 kar1 | This study |
Strain 1735 (MA116-8A) (Lacroute 1971; Aigle and Lacroute 1975) was used as cytoduction donor to BY241 BY241 (Brachmann et al. 2005) forming BEE1021. Segregants of crosses 5168 and 5169 were produced by crossing a strain carrying hsp104-T160M-URA3 (Hung and Masison 2006) twice with BY241 producing strains BEE1200 to BEE1203.