Table 3.
Antibodies used
| Specificity | Animal | Titer | Source |
|---|---|---|---|
| Anti-Btn2 polyclonal | Rabbit | 1:5,000 | custom generated by COVANCE |
| Anti-Cur1 polyclonal | Rabbit | 1:5,000 | custom generated by COVANCE |
| Anti-Sis1 | Rabbit | 1:5,000 | kind gift from Daniel Masison laboratory at NIDDK NIH |
| Anti-HA tag polyclonal | Rabbit | 1:1,000 | Abcam ab 184643 |
| Anti-Pgk1 monoclonal | Mouse | 1:5,000 | Abcam ab 113687 |
| Donkey anti-rabbit IgG-AP | Donkey | 1:5,000 | Santa Cruz Biotechnology sc-2057 used in Figure 2 |
| Anti-rabbit IgG(Fc), AP Conjugate | 1:7,500 | Promega AP conjugate S3738 used in Figure 3 |
Anti-Btn2 and anti-Cur1 antibodies were generated by COVANCE using affinity purified (Ni-NTA agarose, Qiagen) proteins Btn2-His6 and Cur1-His6 produced from pET28-BTN2-HIS and pET28-CUR1-HIS.×