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. 2021 Feb 2;217(4):iyab014. doi: 10.1093/genetics/iyab014

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© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tissue-specific split-wrmScarlet labeling of proteins with distinct subcellular locations. Endogenous proteins tagged with split-wrmScarlet₁₁ in animals expressing split-wrmScarlet_1–10 in somatic tissues, in muscles or in the germline. (A–F) Confocal images of worms expressing somatic split-wrmScarlet_1–10 and (A) EAT-6::split-wrmScarlet₁₁ (plasma membrane), (B) split-wrmScarlet₁₁::TBB-2 (cytoskeleton), (C) split-wrmScarlet₁₁::FIB-1 (nucleoli), (D) HIS-3::split-wrmScarlet₁₁ (nuclei), (E) split-wrmScarlet₁₁::VHA-13 (lysosomes), or (F) TOMM-20::split-wrmScarlet₁₁ (mitochondria). (G) Transgenic worm expressing split-wrmScarlet_1–10 in muscle and split-wrmScarlet₁₁::FIB-1. (H) Transgenic worm expressing split-wrmScarlet_1–10 in the germline and split-wrmScarlet₁₁::FIB-1. (A–H) Maximum intensity projections of 3D stacks shown. Scale bars, 50 µm.