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. 2021 Feb 2;217(4):iyab014. doi: 10.1093/genetics/iyab014

Figure 4.

Figure 4

Split-wrmScarlet11 tandem repeats increase fluorescence. (A) Images of animals carrying either wrmScarlet, split-wrmScarlet11 or two tandem repeats of split-wrmScarlet11 inserted at the endogenous VHA-13 N-terminus. (B) Emission intensities of animals carrying wrmScarlet, split-wrmScarlet11 or dual split-wrmScarlet11 inserted at the VHA-13 N-terminus. Mean ± SD. Circles are individuals. ****P < 0.0001, ***P < 0.001, **P < 0.005. (C) Images of animals carrying either a single split-wrmScarlet11 or three tandem repeats of split-wrmScarlet11 inserted at the HIS-3 C-terminus. (D) split-wrmScarlet emission intensities from animals carrying a single split-wrmScarlet11 or three tandem repeats of split-wrmScarlet11 knock-in at the HIS-3 C-terminus. Mean ± SD. Circles are individuals. ***P < 0.001. Images from each comparison were taken under identical instrument conditions using confocal microscopy and are shown using identical brightness and contrast settings. Images shown are from a single confocal plane. Scale bars, 50 µm.