Figure 12.

Aspartate substitutions in the Gln3_URS abolish Gln3–Tor1 interaction. (A and B) Locations of aspartate substitutions in Gln3 primary and predicted secondary structures of proteins encoded by pRR1124 (Gln3_{R264D, K265K, S267D}) and pRR1128 (Gln3_{K281D, R282D, S285D}). The substitutions in pRR1124 and pRR1128 were the same as those analyzed in pRR754 and pRR772, respectively (Figure 7). Color coding of the Gln3 secondary structure images are the same as in Figures 8 and 9. (C) The two-hybrid experiments were performed and presented as described in Figure 11.