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. 2021 Feb 19;217(4):iyab025. doi: 10.1093/genetics/iyab025

Figure 4.

Figure 4

Altered cellular identity of cohesin variant mESCs. (A) RT-qPCR analysis of pluripotency factors. Levels of Pou5f1 (OCT4), Sox2, and Nanog transcripts were measured in Stag1, Stag2, and Smc1a variant mESCs. n = 4 biological replicates. Significance was determined by Dunnet’s multiple comparisons test; *P < 0.05. (B) RT-qPCR analysis of ectodermal regulators. Levels of Pax6 and Nestin transcripts were measured in Stag1, Stag2, and Smc1a variant mESCs. n = 4 biological replicates. Significance was determined by Dunnet’s multiple comparisons test; *P < 0.05. (C) RT-qPCR analysis of mesodermal regulators. Levels of Foxa2 and T (Brachyury) transcripts were measured in Stag1, Stag2, and Smc1a variant mESCs. n = 4 biological replicates. Significance was determined by Dunnet’s multiple comparisons test; *P < 0.05. (D) RT-qPCR analysis of endodermal regulators. Levels of Gata6 and Sox17 transcripts were measured in Stag1, Stag2, and Smc1a variant mESC lines. n = 4 biological replicates. Significance was determined by Dunnet’s multiple comparisons test; *P < 0.05.