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. 2021 Feb 10;18:28–39. doi: 10.2142/biophysico.bppb-v18.004

Figure 2 .

Figure 2 

Some limitations of PCR. (a) Non-specific (i.e., false-positive) amplifications are easily obtained by the effects of other viruses, bacteria, and contaminations in a laboratory. (b) The handling volume for PCR is usually a μL order, possibly failing to capture the target nucleic acids (see the left panel). If it becomes a 100 μL order or more, like that of a microplate reader well, the chance of capturing the target nucleic acids becomes larger (see the right panel).