Table 1 .
Ingredients of 5× LB broth
5× LB broth | 1× concentration | |
---|---|---|
bacto-tryptone | 50.0 g | 10.0 g/L |
bacto-yeast extract | 25.0 g | 5.0 g/L |
NaCl | 50.0 g | 10.0 g/L |
NaOH | adjust to pH 7.5 | |
D. W | up to 1 L | |
Total | 1 L | *autoclave |
Ingredients of 5× LB broth
5× LB broth | 1× concentration | |
---|---|---|
bacto-tryptone | 50.0 g | 10.0 g/L |
bacto-yeast extract | 25.0 g | 5.0 g/L |
NaCl | 50.0 g | 10.0 g/L |
NaOH | adjust to pH 7.5 | |
D. W | up to 1 L | |
Total | 1 L | *autoclave |