Table 1 .
Ingredients of 5× LB broth
| 5× LB broth | 1× concentration | |
|---|---|---|
| bacto-tryptone | 50.0 g | 10.0 g/L |
| bacto-yeast extract | 25.0 g | 5.0 g/L |
| NaCl | 50.0 g | 10.0 g/L |
| NaOH | adjust to pH 7.5 | |
| D. W | up to 1 L | |
| Total | 1 L | *autoclave |
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