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Ingredients of 30%, 60% and 80% D2O LB broth
| 30% D2O LB broth | |
|---|---|
| 5× LB broth | 1.0 mL |
| filter-sterilized D2O | 1.5 mL |
| sterile H2O | 2.5 mL |
| antibiotic (in H2O) | |
| Total | 5.0 mL |
| 60% D2O LB broth | |
| 5× LB broth | 1.0 mL |
| filter-sterilized D2O | 3.0 mL |
| sterile H2O | 1.0 mL |
| antibiotic (in H2O) | |
| Total | 5.0 mL |
| 80% D2O LB broth | |
| 5× LB broth | 1.0 mL |
| filter-sterilized D2O | 4.0 mL |
| antibiotic (in H2O) | |
| Total | 5.0 mL |
