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Ingredients of 100% D2O M9 medium
| Ingredient | weight/volume | final concentration |
|---|---|---|
| NH4Cl | 1.0 g | 18.7 mM |
| KH2PO4 | 2.0 g | 14.7 mM |
| Na2HPO4 | 6.34 g | 44.7 mM |
| NaCl | 0.5 g | 8.6 mM |
| deuterated D-glucose | 2.0 g | 2.0 mg/mL |
| D2O | 1 L | |
| freeze-dried antibiotic | appropriate weight | appropriate concentration |
| freeze-dried additive | Refer to Table 3 | |
| Total | 1 L | *filter sterilize |
