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Ingredients of 75% D2O M9 medium
| Ingredient | weight/volume | final concentration |
|---|---|---|
| 10× M9 medium | 100.0 mL | |
| deuterated D-glucose | 1.5 g | 1.5 mg/mL |
| hydrogenated D-glucose | 0.5 g | 0.5 mg/mL |
| D2O | 750.0 mL | |
| H2O | 150.0 mL | |
| freeze-dried antibiotic | appropriate weight | appropriate concentration |
| freeze-dried additive | Refer to Table 3 | |
| Total | 1 L | *filter sterilize |
