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Steady-state affinity ‘KD’ and the maximum response “Rmax’ were estimated from non-linear regression by fitting the equilibrium binding data to steady-state affinity equation. ND- not determined.
| Enzyme | Steady-state affinity ‘KD’ (¼M) | Rmax (RU) | ||
|---|---|---|---|---|
| Enzyme | FPP | GPP | FPP | GPP |
| WT-LmFPPS | 36.6 | 0.049 | 20.3 | 4.1 |
| E97Y-LmFPPS | 36.4 | 1.27 | 28.5 | 4.3 |
| T164Y-LmFPPS | ND | ND | ND | ND |
| T164W-LmFPPS | ND | ND | ND | ND |
