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. 2021 Mar 13;40(15):2725–2740. doi: 10.1038/s41388-021-01740-6

Fig. 5. LDHA knockdown in POU1F-overexpressing cancer cells reduces tumor growth, and tumor glucose uptake in vivo.

Fig. 5

A Body weight of mice orthotopically injected with MCF7, MCF7-POU1F1, and MCF7-POU1F1-shLDHA human breast cancer cells (n = 7 mice per group). B Tumor volume of mice injected with MCF7, MCF7-POU1F1, and MCF7-POU1F1-shLDHA cells and representative images of tumors. Scale bar: 1000 μm. C qWB analysis of POU1F1, LDHA and β-actin in tumors from nine mice injected with MCF7 (n = 3), MCF7-POU1F1 (n = 3) and MCF7-POU1F1-shLDHA cells (n = 3). D ki67 immunostaining of mouse tumors. Scale bar: 50 μm. E On the left, SUVmax values for the three experimental groups (n = 4 mice per group). Significant differences can be observed between MCF7-POU1F1 and other groups (*P < 0.05, **P < 0.01). On the right, [18F]FDG PET/CT images from representative BALB/c-nu mice. Metabolic activity is coded on a color scale ranging from blue (low [18F]FDG uptake) to red (high [18F]FDG uptake). At the top, xenograft tumors indicated by arrows on CT images. At the bottom, tumors delineated using PET/CT images. Axial views are also shown. F Correlation between POU1F1 and LDHA mRNA expression and relapse-free survival (RFS) in human breast tumors (n = 3951). G Correlation between POU1F1 and LDHA mRNA expression and overall survival (OS) in human breast tumors (n = 42). Analysis of (F) and (G) were done using the KM plotter and the ProgGeneV2 online tools, respectively.