Fig. 4.

αKlotho downregulates the serum-stimulated cell surface abundance of Orai1. a Cell-surface biotinylation assay showing the effect of αKlotho on the serum-stimulated cell-surface abundance of Orai1. b Quantification of the results in panel a. **p < 0.01 vs. serum deprivation and #p < 0.01 vs. serum incubation. c Representative SOCE traces showing that αKlotho suppressed SOCE, and prevented the inhibition by Brefeldin A (BFA). Cells were preincubated with BFA (10 μM for 8 h) before αKlotho treatment (1 h). d Summary of SOCE in panel c (n = 53–252 for each). **p < 0.01 vs. vehicle control (no αKlotho). NS, not significant between each group. e Representative SOCE traces show that αKlotho reduced SOCE and prevented the suppression by tetanus toxin (TeNT, 60 nM, 16 h). f Summary of SOCE in panel e (n = 84–241 for each). **p < 0.01 vs. vehicle control (no αKlotho). NS, not significant between each group. One-way ANOVA in b, d, and f