| Component | Amount (μL) | Final |
|---|---|---|
| Nuclease-free water | to 10 μL | |
| KAPA SYBR FAST qPCR Master Mix (2X) | 10 | 1X |
| PBLib-preamp-F1, 10 μM | 0.8 | 0.4 μM |
| PBLib-preamp-R1, 10 μM | 0.8 | 0.4 μM |
| Genomic DNA | 6 pg – 0.2 ng | 6 pg – 0.2 ng |
| Total volume | 20 |
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