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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Protoc. 2021 Mar 10;16(4):2088–2108. doi: 10.1038/s41596-020-00485-y
Component Amount (μL) Final
Nuclease-free water to 10 μL
KAPA SYBR FAST qPCR Master Mix (2X) 5 1X
PBLib-F primer mix, 10 μM 0.25 0.25 μM
PBLib-R primer mix, 10 μM 0.25 0.25 μM
Genomic DNA
or
Diluted PCR#0 product		 2 0.2 ng – 10 ng
or
N/A
Total volume 10