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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Protoc. 2021 Mar 10;16(4):2088–2108. doi: 10.1038/s41596-020-00485-y

Table 2.

Primer mix compositions for PCR#1.

Primer Mix Primer μM in primer mix
PBLib-F (10 μM) SBS3-PBLib-F 2.5
trSBS3 7.5
PBLib-R (10 μM) SBS9-PBLib-R 1.67
trSBS9 7.5
SBS9-PBLib30-R 0.83