Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Small. 2020 Oct 23:e2004258. doi: 10.1002/smll.202004258

Figure 2. Engineering of BC tissue models where breast spheroids are encapsulated in hydrogels mimicking mechanical properties of native breast tissues.

Figure 2.

a) Schematic of the procedure to make physiologically relevant BC tissue models. b) BC spheroids cultured for 1 and 3 days in the microwells. c) Histogram of the spheroid dimeters after collecting them from the microwells on day 5 (N=80). d) Schematic illustration of measurement of the compressive elastic modulus of GelMA hydrogels with or without a presence of BC spheroids. e) Stress-strain curves of GelMA with or without BC spheroids. f) Measured compressive elastic modulus of GelMA with or without BC spheroids (N=3). g) Representative immunofluorescence images of live/dead staining for BC cells in spheroids after 1 and 5 days in culture. h) Proliferation index for BC cells in spheroids cultured for 5 days in GelMA hydrogels (N=3). i) Representative immunofluorescence images of DAPI/HER-2 staining of spheroids on day 5 after culturing in GelMA hydrogels. (One-way ANOVA with Tukey significant difference post-hoc test; **p<0.005). Error bars represent standard deviation.